TRP-PLIK, a Bifunctional Protein with Kinase and Ion Channel Activities
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- 9 February 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 291 (5506) , 1043-1047
- https://doi.org/10.1126/science.1058519
Abstract
We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing α-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.Keywords
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