Abstract
Benzoic acid in concentrations from 0.18 per cent and upwards exerted a bactericidal action on the gram‐negative flora of dialysis fluid at pH 4.75 in vitro. At a concentration of 0.32 per cent, a reduction of 105 was obtained within 24 hours. Sulphonamide resistance did not affect the bactericidal action. The killing effect was hardly more than directly proportional to the concentration of benzoic acid. Increasing inoculum decreased the bactericidal effect. Therefore, benzoic acid should not be used as an artificial kidney disinfectant when the concentration of microorganisms exceeded 105.5/0.1 ml.

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