Genetic Variation in the Human Insulin Gene
- 1 August 1980
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 209 (4456) , 612-615
- https://doi.org/10.1126/science.6248962
Abstract
Four recombinant lambda phages containing nucleotide sequences complementary to a cloned human preproinsulin DNA probe have been isolated from human DNA. Restriction analyses in conjunction with Southern hybridizations reveal two types of gene sequences. One isolate of each type was subjected to complete nucleotide sequence determination. The sequences contain the entire preproinsulin messenger RNA region, two intervening sequence. 260 nucleotides upstream from the messenger RNA capping site, and 35 nucleotides beyond the polyadenylate attachment site. Our results strongly suggest that these two gene types are allelic variants of a single insulin gene.Keywords
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