Transformation of chlorinated phenolic compounds in the genusRhodococcus
- 1 September 1989
- journal article
- research article
- Published by Springer Nature in Microbial Ecology
- Vol. 18 (2) , 147-159
- https://doi.org/10.1007/bf02030123
Abstract
The ability of strains of the genusRhodococcus to transform chlorinated phenolic compounds was studied. Noninduced cells of several strains ofRhodococcus, covering at least eight species, were found to attack mono-, di-, and trichlorophenols by hydroxylation at theortho position to chlorocatechols. 3-chlorophenol and 4-chlorophenol were converted to 4-chlorocatechol, 2,3-dichlorophenol to 3,4-dichlorocatechol, and 3,4-di-chlorophenol to 4,5-dichlorocatechol. The chlorocatechols accumulated to nearly stoichiometric amounts. Other mono- and dichlorophenols were not transformed. The ability of the strains to hydroxylate chlorophenols correlated with the ability to grow on unsubstituted phenol as the sole source of carbon and energy. SeveralRhodococcus strains attacked chlorophenolic compounds by both hydroxylation and O-methylation. 2,3,4-, 2,3,5- and 3,4,5-trichlorophenol were hydroxylated to trichlorocatechol and then sequentially O-methylated to chloroguaiacol and chloroveratrole. Tetrachlo-rohydroquinone was O-methylated sequentially to tetrachloro-4-methoxy-phenol and tetrachloro-1,4-dimethoxybenzene. Several of the active strains had no known history of exposure to any chloroaromatic compound. Rhodococci are widely distributed in soil and sludge and these results suggest that this genus may play an important role in transformation of chlorinated phenolic compounds in the environment.This publication has 44 references indexed in Scilit:
- Critical steps in degradation of chloroaromatics by rhodococci IV. Detailed kinetics of substrate removal and product formation by resting pre‐adapted cellsJournal of Basic Microbiology, 1989
- Critical steps in degradation of chloroaromatics by rhodococci III. Isolation and identification of accumulating intermediates and deadend productsJournal of Basic Microbiology, 1989
- Critical steps in degradation of chloroaromatics by rhodococci II. Whole‐cell turnover of different monochloroaromatic non‐growth substrates by Rhodococcus sp. An 117 and An 213 in the absence/presence of glucoseJournal of Basic Microbiology, 1988
- Contamination of soil around wood-preserving facilities by polychlorinated aromatic compoundsEnvironmental Science & Technology, 1987
- Migration of wood-preserving chemicals in contaminated groundwater in a sand aquifer at Pensacola, FloridaEnvironmental Science & Technology, 1985
- Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylaseFEBS Letters, 1984
- Methylation and Hydroxylation of Pentachiorophenol by Mycobacterium sp. Isolated from SoilJournal of Pesticide Science, 1983
- The Purification and Properties of 2,4‐Dichlorophenol Hydroxylase from a Strain of Acinetobacter SpeciesEuropean Journal of Biochemistry, 1982
- DEGRADATION OF AROMATIC COMPOUNDS IN THE ACTINOMYCETE-GENUSRHODOCOCCUSFEMS Microbiology Letters, 1980
- Cometabolism of phenol and substituted phenols bynocardiaspec. DSM 43251FEMS Microbiology Letters, 1979