Crystallization of nerve growth factor from mouse submaxillary glands.
- 1 March 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (3) , 777-779
- https://doi.org/10.1073/pnas.72.3.777
Abstract
Crystals of the nerve growth factor protein were grown by vapor diffusion from ethanol solution. The crystals are hexagonal, belonging to space group P622 (or its enantiomorph) with a equals 56.1 A, c equals 181.4 A, and V equals 494,400 A. The unit cell contains six molecules of dimeric protein and thus has one monomer per asymmetric unit. The diffraction pattern extends to at least 2.7 A, indicating that this crystal form is suitable for structural analysis to near-atomic resolution.Keywords
This publication has 12 references indexed in Scilit:
- Solvent content of protein crystalsPublished by Elsevier ,2006
- Determination of molecular weight from protein crystalsJournal of Molecular Biology, 1974
- Topography of mouse 2.5S nerve growth factor. Reactivity of tyrosine and tryptophanBiochemistry, 1973
- Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structureBiochemistry, 1973
- The Physical and Biological Properties of 7S and β-NGF from the Mouse Submaxillary GlandPublished by Springer Nature ,1972
- Nerve Growth Factor from Mouse Submaxillary Gland: Amino Acid SequenceProceedings of the National Academy of Sciences, 1971
- THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEINProceedings of the National Academy of Sciences, 1969
- Nerve growth factor.Physiological Reviews, 1968
- The Isolation of the Mouse Nerve Growth Factor Protein in a High Molecular Weight Form*Biochemistry, 1967
- PURIFICATION OF A NERVE-GROWTH PROMOTING PROTEIN FROM THE MOUSE SALIVARY GLAND AND ITS NEURO-CYTOTOXIC ANTISERUMProceedings of the National Academy of Sciences, 1960