Use of an Established Cat Cell Line for Investigation and Quantitation of Feline Oncornaviruses

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Abstract
Susceptibility of a continuous cat kidney cell line of Crandell (CCC) to focus formation by a feline leukemia pseudotype of murine sarcoma virus, MSV(FeLV), was compared to that of feline embryonic fibroblast (FEF) cultures. CCC appeared to be nearly as susceptible to infection with MSV(FeLV) as FEFi virus titers on CCC were only three-fold lower than those on FEF. The MSV(FeLV) stock showed a characteristic “two-hit” titration pattern when assayed in CCC as well as in FEF with or without addition of diethylaminoethyl (DEAE)-dextran (D) to the assay plates. In the presence of added FeLV, the number of foci formed by MSV(FeLV) was increased and the titration pattern became essentially “one-hit.” Pretreatment of both CCC and FEF cultures with DEAE-D enhanced by threefold to four-fold the focus formation by MSV(FeLV) either alone or with exogenous FeLV. Preinfection of CCC cultures with FeLV or the Gardner-Arnstein strain of feline sarcoma virus induced resistance to challenge with MSV(FeLV). In addition, colonies of transformed cells were produced in semisolid agar suspension cultures after infection of CCC with MSV(FeLV).

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