Sulfonamide resistance mechanism in Escherichia coli: R plasmids can determine sulfonamide-resistant dihydropteroate synthases.
- 1 July 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (7) , 2621-2625
- https://doi.org/10.1073/pnas.72.7.2621
Abstract
Several natural isolate E. coli strains highly resistant to sulfonamides and antibiotics are shown to contain a sulfonamide-resistant dihydropteroate synthase (2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine-diphosphate:4-aminobenzoate 2-amino-4-hydroxydihydropteridine-6-methenyltransferase, EC 2.5.1.15) in addition to the normal sensitive enzyme. The resistant dihydropteroate synthases examined are determined by an R plasmid and are smaller and less heat stable than the normal sulfonamide-sensitive enzyme. One synthase resistant to any sulfonamide tested, and to sulfanilic and arsanilic acids, was still inhibited by several non-sulfonamide analogs of p-aminobenzoate. Citrobacter and Klebsiella pneumoniae strains also show similar mechanisms of sulfonamide resistance.Keywords
This publication has 23 references indexed in Scilit:
- A New Dihydrofolate Reductase with Low Trimethoprim Sensitivity Induced by an R Factor Mediating High Resistance to TrimethoprimJournal of Biological Chemistry, 1974
- Molecular structure of bacterial plasmids.1972
- Enzymic synthesis of hydroxymethyldihydropteridine Pyrophosphate and dihydrofolateBiochemistry, 1969
- The biosynthesis of folic acid. IX. Purification and properties of the enzymes required for the formation of dihydropteroic acid.1969
- The Enzymatic Synthesis of Dihydrofolate and Dihydropteroate in Cell-Free Preparations from Wild-Type and Sulfonamide-Resistant Pneumococcus*Biochemistry, 1966
- INHIBITOR BINDING ANALYSIS OF DIHYDROFOLATE REDUCTASES FROM VARIOUS SPECIES1965
- The Enzymatic Synthesis of Folate-like Compounds from Hydroxymethyldihydropteridine PyrophosphateJournal of Biological Chemistry, 1964
- 5-Benzyl-2,4-diaminopyrimidines as Antibacterial Agents. I. Synthesis and Antibacterial Activity in vitroJournal of Medicinal Chemistry, 1962
- Analysis of the Biochemical Mechanism of Drug Resistance in Certain Bacterial MutantsProceedings of the National Academy of Sciences, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951