INVIVO AND INVITRO EFFECTS OF PEPTIDASE EXTRACTS FROM AMANITA-VIROSA

  • 1 January 1982
    • journal article
    • research article
    • Vol. 41  (1) , 23-34
Abstract
Cyclic peptides from the deadly mushroom A. virosa were separated by methanolic extraction and chromatography on Sephadex. Three groups of peptides were obtained, virotoxins, amaninamide and phalloidin. Virotoxins were separated in 2 fractions virotoxins A and virotoxins B. The properties of these 2 fractions were studied on F actin in vitro and on mice in vivo. Virotoxins A and B protected F actin in vitro against chaotropic ions, depolymerization by DNAse or cytochalasin B and heat denaturation. Virotoxins A and B increased the rate of polymerization of F actin. Virotoxins A and B were toxic compounds which produce hemorragic necrosis of liver observed in detail by EM. In vitro and in vivo results showed that virotoxins exhibited the same effect as phalloidin on F actin.

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