Efficient splicing of an AU-rich antisense intron sequence
- 1 January 1993
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 21 (2) , 205-211
- https://doi.org/10.1007/bf00019937
Abstract
For successful splicing in dicot plants the only recognised intron requirements are 5' and 3' splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.Keywords
This publication has 29 references indexed in Scilit:
- Rapid analysis of plant gene expression by a novel reverse transcriptase‐PCR methodThe Plant Journal, 1992
- Pre-mRNA splicing in yeastTrends in Genetics, 1991
- Expression of β amyloid protein precursor mRNAs: Recognition of a novel alternatively spliced form and quantitation in alzheimer's disease using PCRNeuron, 1990
- Hierarchy for 5′ splice site preference determined in vivoJournal of Molecular Biology, 1990
- The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicingCell, 1989
- Reverse self-splicing of the tetrahymena group I intron: Implication for the directionality of splicing and for intron transpositionCell, 1989
- The Maize Transposable Element Ds Is Spliced from RNAScience, 1987
- Implications of RNA⋅RNA Splicing in Evolution of Eukaryotic CellsScience, 1978
- Genes in pieces: were they ever together?Nature, 1978
- Why genes in pieces?Nature, 1978