An NADH-Dependent Acetoacetyl-CoA Reductase from Euglena gracilis
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 82 (4) , 978-984
- https://doi.org/10.1104/pp.82.4.978
Abstract
An NADH-dependent acetoacetyl-CoA reductase from Euglena gracilis variety bacillaris was extensively purified and characterized. Two different isoelectric forms of the reductase with identical characteristics otherwise were found. The reductase was noncompetitively inhibited by acyl carrier protein, Ki 5.6 micromolar at pH 5.4; this inhibition decreased with increasing pH or ionic strength. Coenzyme A was a competitive inhibitor, Ki 230 micromolar. Kinetic parameters with respect to acetoacetyl-CoA and NADH were sensitive to changes in pH and ionic strength.This publication has 23 references indexed in Scilit:
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