An Endosomal‐Lysosomal Pathway for Degradation of Amyloid Precursor Protein a
- 17 December 1992
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 674 (1) , 103-117
- https://doi.org/10.1111/j.1749-6632.1992.tb27480.x
Abstract
We previously reported evidence for a lysosomal degradative pathway for APP and C-terminal fragments thereof, based on Western and immunocytochemical analysis of drug-treated cells. Here, we verify the existence of a lysosomal degradative pathway for APP using pulse chase immunoprecipitation analysis of drug-treated cells and fibroblasts with and without a known lysosomal hydrolase targeting defect. The results are consistent with the hypothesis that part or all of the beta-protein domain of APP is normally degraded by lysosomes. A mechanism for beta-protein deposition based on this data is hypothesized.Keywords
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