A Simple Prfparation Technioue for a Microsomal Na+K+Activated at Pase from Cardiac Tisstlfs of Different Specifs
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 4 (1) , 13-29
- https://doi.org/10.1080/00327487408068183
Abstract
A simple and relatively gentle technique for Isolation of a cardiac microsomal Na+ - K+- adenoslnetrIphosphatase (Na+ - K+-ATPase) Is described, consisting of one differential centrlfugatIon step followed by gel chromatography (Sepharose 4B) of the supernatant. Depending on the species (rat, guinea pig, rabbit), the preparations contained 2 to 4% of the total protein, while the mitochondrial contamination (as estimated by succinatedehydrogenase (SDH) - activity) was In the range of 2 to 10 % of the initial content. The Na+- K+ - ATPase activity was between 10 and 40 μmol P1/mg protein/hour, corresponding to 30 to 45 % of the total activity. Further, an automatic assay for the determination of ATPase activities Is described.Keywords
This publication has 11 references indexed in Scilit:
- Species and tissue differences in the rate of dissociation of ouabain from (Na+ + K+)-ATPaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Direct Method for Determining Inorganic Phosphate in Serum with the "CentrifiChem"Clinical Chemistry, 1972
- Micro estimation of RNA by the cupric ion catalyzed orcinol reactionAnalytical Biochemistry, 1969
- ULTRASTRUCTURAL STUDIES ON THE PERMEABILITY OF THE MESOTHELIUM TO HORSERADISH PEROXIDASEThe Journal of cell biology, 1968
- On the Mechanism of Na+‐and K+‐Stimulated Hydrolysis of Adenosine TriphosphateEuropean Journal of Biochemistry, 1967
- Die verschiedene Empfindlichkeit der Na+ + K+-aktivierten ATPase des Herz- und Skeletmuskels gegen k-StrophanthinNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1966
- THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- Isolation of skeletal muscle cell membrane and some of its propertiesArchives of Biochemistry and Biophysics, 1961
- Staining of Tissue Sections for Electron Microscopy with Heavy MetalsThe Journal of cell biology, 1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951