Effects of riboflavin and selenium deficiencies on glutathione and its relating enzyme activities with respect to lipid peroxide content of rat livers.
- 1 January 1983
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 29 (3) , 283-292
- https://doi.org/10.3177/jnsv.29.283
Abstract
Weanling male rats were fed a riboflavin- or Se-deficient diet for 5 wk, and the glutathione content and its relating enzyme activites in the livers were examined. The glutathione content and glutathione reductase activity were decreased by deficiency of riboflavin, but not by that of Se. Glutathione peroxidase activity was increased by addition of Se to the diet, but without its addition, the activity was higher in the riboflavin-deficient rats than in the riboflavin-sufficient rats, in spite of the increase of lipid peroxides in the former rats. G-6-P dehydrogenase activity was decreased significantly by riboflavin deficiency. In another experiment, riboflavin was i.p. injected into rats fed the diet to which neither riboflavin nor Se were added. The glutathione content and activities of glutathione reductase and G-6-P dehydrogenase returned to the control level of riboflavin-sufficient rats in 24 h, the lipid peroxide level in 48 h and the glutathione peroxidase activity, being higher than that in the control rats, in 72 h after the injection, respectively. The increase of lipid peroxides in the livers of riboflavin-deficient rats is caused by the decrease in the glutathione content as well as glutathione reductase activity rather than by that in the Se-dependent glutathione peroxidase activity.This publication has 13 references indexed in Scilit:
- Glutathione peroxidase activity in selenium-deficient rat liverPublished by Elsevier ,2004
- Rat hepatic cytosolic glutathione-dependent enzyme protection against lipid peroxidation in the nadph-microsomal lipid peroxidation systemBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- Effects of riboflavin deficiency on lipid peroxidation of rat liver microsomes.Journal of Nutritional Science and Vitaminology, 1980
- The mechanism of NADPH-dependent lipid peroxidation. The propagation of lipid peroxidation.Journal of Biological Chemistry, 1979
- Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase.Journal of Biological Chemistry, 1978
- Immunochemical Study on the Pathway of Electron Flow in Reduced Nicotinamide Adenine Dinucleotide-Dependent Microsomal Lipid Peroxidation1The Journal of Biochemistry, 1978
- Lipid Peroxidation in Vivo during Vitamin E and Selenium Deficiency in the Rat as Monitored by Ethane EvolutionJournal of Nutrition, 1977
- Rat Liver Glutathione: Possible Role as a Reservoir of CysteineJournal of Nutrition, 1977
- Effect of glutathione peroxidase activity on lipid peroxidation in biological membranesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951