Fixing and Staining of Conifer Tissues with Lacto-Propiono-Dimethylsulfoxide Carmine
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 51 (3) , 197-199
- https://doi.org/10.3109/10520297609116699
Abstract
During a study of the development of callus from immature microsporophylls and megagametophytes of Pinus spp. (Bonga 1974), tissue squashes were stained with aceto-carmine (Gerlach 1969). However, this stain had some drawbacks. The cells and nuclei formed in the pollen and pollen tubes during the early stages of culture were often obscured by large numbers of granules (cf. Christiansen 1973), and often, small groups of small vigorous cells within the callus failed to stain.This publication has 1 reference indexed in Scilit:
- In vitro culture of microsporophylls and megagametophyte tissue of pinusIn Vitro Cellular & Developmental Biology - Plant, 1974