Nucleotide Sequence of the -Lactamase Gene of Alkalophilic Bacillus sp. Strain 170
- 1 December 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (12) , 3317-3324
- https://doi.org/10.1099/00221287-131-12-3317
Abstract
Summary: A gene for the β-lactamase from alkalophilic Bacillus sp. strain 170 was cloned in a functional state on a 1-0 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame of 257 amino acids, which represents the β-lactamase precursor protein. It is considered that the signal peptide consisted of 30 amino acids including 12 hydrophobic amino acids.Keywords
This publication has 12 references indexed in Scilit:
- Translational block to expression of the Escherichia coli Tn9-derived chloramphenicol-resistance gene in Bacillus subtilis.Proceedings of the National Academy of Sciences, 1982
- Membrane-bound penicillinases in Gram-positive bacteria.Journal of Biological Chemistry, 1982
- The promoter-proximal region of the Bacillus licheniformis penicillinase gene: Nucleotide sequence and predicted leader peptide sequenceGene, 1981
- Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.Proceedings of the National Academy of Sciences, 1981
- Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformisBiochemistry, 1980
- Simple method for identification of plasmid-coded proteinsJournal of Bacteriology, 1979
- Membrane-bound and secreted forms of penicillinase from Bacillus licheniformisJournal of Molecular Biology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977
- Production of Penicillinase by an AlkalophilicBacillusAgricultural and Biological Chemistry, 1976