New Types of Gangliosides in Starfish with Sialic Acid Residues in the Inner Part of Their Carbohydrate Chains
- 1 September 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 80 (3) , 637-640
- https://doi.org/10.1093/oxfordjournals.jbchem.a131320
Abstract
Three gangliosides were isolated from the starfish by Folch's partition, dialysis, silicic acid column chromatography, Iatrobead column chromatography and preparative thin-layer chromatography. Partial acid hydrolysis of the three lipids in 0.1 N HCl at 80° for 90 min liberated all the lactosyl ceramide and water-soluble degradation products, but no sialic acid. Gas-liquid chromatography of the trimethylsilylated methyl glycosides of the water-soluble products demonstrated the presence of arabinose in addition to the usual sugar units of gangliosides.This publication has 3 references indexed in Scilit:
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- Quantitive estimation of sialic acidsBiochimica et Biophysica Acta, 1957
- THE DETERMINATION OF SUGAR IN BLOOD AND SPINAL FLUID WITH ANTHRONE REAGENTJournal of Biological Chemistry, 1955