Requirement for p53 and p21 to Sustain G 2 Arrest After DNA Damage
- 20 November 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 282 (5393) , 1497-1501
- https://doi.org/10.1126/science.282.5393.1497
Abstract
After DNA damage, many cells appear to enter a sustained arrest in the G 2 phase of the cell cycle. It is shown here that this arrest could be sustained only when p53 was present in the cell and capable of transcriptionally activating the cyclin-dependent kinase inhibitor p21. After disruption of either the p53 or the p21 gene, γ radiated cells progressed into mitosis and exhibited a G 2 DNA content only because of a failure of cytokinesis. Thus, p53 and p21 appear to be essential for maintaining the G 2 checkpoint in human cells.Keywords
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