SUBMICROSCOPIC PARTICLES IN EXTRACTS OF AZOTOBACTER AGILIS

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Abstract
Sonic extracts contain 2 types of submicroscopic particles which were separated by electrophoresis on starch. One particle is contained in the cytoplasm and consists of ribonucleoprotein; it is about 30 mu in diameter and has a sedimentation constant of 42 S. A second particle is derived by fragmentation of the cell envelope. It contains hydrogenase, cytochrome and phospholipid and is responsible for oxidative phosphorylation. The cell envelope or hull is obtained by brief sonic treatment. Purified hulls contain hydrogenase, cytochrome, phospholipid and the necessary enzymes for oxidative phosphorylation.
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