Cyclic AMP controls the plasma membrane H+‐ATPase activity from Saccharomyces cerevisiae

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Abstract
The thermosensitive G1‐arrested cdc35‐10 mutant from Saccharomyces cerevisiae, defective in adenylate cyclase activity, was shifted to restrictive temperature. After 1 h incubation at this temperature, the plasma membrane H+‐ATPase activity of cdc35‐10 was reduced to 50%, whereas that in mitochondria doubled. Similar data were obtained with cdc25, another thermosensitive G1‐arrested mutant modified in the cAMP pathway. In contrast, the ATPase activities of the G1‐arrested mutant cdc19, defective in pyruvate kinase, were not affected after 2 h incubation at restrictive temperature. In the double mutants cdc35‐10 cas1 and cdc25 cas1, addition of extracellular cAMP prevented the modifications of ATPase activities observed in the single mutants cdc35‐10 and cdc25. These data indicate that cAMP acts as a positive effector on the H+‐ATPase activity of plasma membranes and as a negative effector on that of mitochondria.