Oxygen exchange between the Fe(IV)O heme and bulk water for the A2 isozyme of horseradish peroxidase
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 305-307
- https://doi.org/10.1016/0014-5793(86)81038-9
Abstract
Resonance Raman spectra were observed for compound II of horseradish peroxidase A2, and the Fe(IV)O stretching Raman line was identified at 775 cm−1. This Raman line shifted to 741 cm−1 upon a change of solvent from H2 16O to H2 18O, indicating occurrence of the oxygen exchange between the Fe(IV)O heme and bulk water. The oxygen exchange took place only at the acidic side of the heme‐linked ionization with pK a = 6.9.Keywords
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