BIOLOGICAL-ACTIVITIES OF TRITIATED ENDOTOXINS - CORRELATION OF LIMULUS LYSATE ASSAY WITH RABBIT PYROGEN AND COMPLEMENT-ACTIVATION ASSAYS FOR ENDOTOXIN

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (2) , 308-315
Abstract
Tritiated [Escherichia coli] endotoxins were prepared by 3 different methods. The biological activities of the tritiated endotoxins were determined by the Limulus amebocyte lysate assay, a rabbit pyrogen assay and a complement-activation assay and were compared to native, unlabeled endotoxin. All 3 tritiated endotoxin preparations manifested adequate biological activity in each of the 3 assay systems, and all 3 assays ranked the biological activity of the different endotoxin preparations in the same order. Endotoxin tritiated by the Wilzbach procedure retained most of its biological activity and also had the highest specific radioactivity. The good correlation between the Limulus lysate, rabbit pyrogen and complement-activation assays suggests that the same active site of the endotoxin molecule is identified by the 3 different assays.

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