Fluorometric Determination of Residual Sulfisomezole in Animal Tissues with Fluorescamine

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Abstract
Reaction conditions for the fluorometric determination of sulfisomezole with fluorescamine were examined, and analytical methods for sulfisomezole were established. These methods were applied to the measurements of 0.1 ppm level of sulfisomezole in animal tissue, and the content of sulfisomezole and total sulfisomezole in egg yolk, egg albumen, chicken tissues (kidney, liver, heart, gizzard, spleen, muscle, skin and fat), and pig tissues (kidney, liver, heart, stomach, spleen, muscle and duodenum) were determined. Sulfisomezole in these tissues was extracted with AcOEt [ethyl acetate], CH2Cl2 or C2H4Cl2. These methods are simpler and 10 times more sensitive than the colorimetric method using Bratton-Marsall or Tsuda reagent, and amino acids and aliphatic amines do not interfere. These methods were also used to trace the quantity of residual sulfisomezole in pig tissues after i.m. injection or oral administration.

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