Identification of a Cyclase Gene Dictating the C-9 Stereochemistry of Anthracyclines from Streptomyces nogalater
- 1 February 2000
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 44 (2) , 396-399
- https://doi.org/10.1128/aac.44.2.396-399.2000
Abstract
Nogalamycin is an anthracycline antibiotic produced by Streptomyces nogalater . Its aglycone has a unique stereochemistry (7S, 9S, 10R) compared to that of most other anthracyclines (7S, 9R, 10R). The gene sno aL, encoding a nogalonic acid methyl ester cyclase for nogalamycin, was used to generate nogalamycinone, demonstrating that the single cyclase dictates the C-9 stereochemistry of anthracyclines.Keywords
This publication has 20 references indexed in Scilit:
- Elucidation of anthracyclinone biosynthesis by stepwise cloning of genes for anthracyclines from three different Streptomyces spp. The GenBank accession number for acmA reported in this paper is AF043550.Microbiology, 2000
- Characterization of Streptomyces nogalater genes encoding enzymes involved in glycosylation steps in nogalamycin biosynthesisMolecular Genetics and Genomics, 1997
- Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesisJournal of Bacteriology, 1997
- Production of hybrid anthracycline antibiotics by heterologous expression of Streptomyces nogalater nogalamycin biosynthesis genesMicrobiology, 1996
- Isolation and characterization of aclacinomycin A-non-producing Streptomyces galilaeus (ATCC 31615) mutantsMicrobiology, 1994
- Codon usage in the G+C-rich Streptomyces genomeGene, 1992
- Molecular Structure, Stereochemistry and Interactions of Steffimycin B, and DNA Binding Anthracycline AntibioticJournal of Biomolecular Structure and Dynamics, 1985
- Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeusGene, 1985
- Molecular structure, absolute stereochemistry, and interactions of nogalamycin, a DNA-binding anthracycline antitumor antibioticJournal of the American Chemical Society, 1983
- Structure of nogalamycinJournal of the American Chemical Society, 1977