Ku complex controls the replication time of DNA in telomere regions
Open Access
- 1 October 2002
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 16 (19) , 2485-2490
- https://doi.org/10.1101/gad.231602
Abstract
We have investigated whether the Ku complex is involved in regulating DNA replication in the yeast Saccharomyces cerevisiae. We find that Ku proteins control the replication time of telomeric regions; replication origins located close to telomeres or within subtelomeric repeat sequences normally initiate late, but are activated much earlier in mutants lacking Ku function. In contrast, origins distant from telomeres initiate replication at the normal time. Ku is one of the first components identified as important for replication timing, and specification of the replication time of chromosome ends by Ku is consistent with its role in maintaining telomere localization.Keywords
This publication has 38 references indexed in Scilit:
- Genome-Wide Distribution of ORC and MCM Proteins in S. cerevisiae : High-Resolution Mapping of Replication OriginsScience, 2001
- In Vivo Association of Ku with Mammalian Origins of DNA ReplicationMolecular Biology of the Cell, 2001
- Replication Dynamics of the Yeast GenomeScience, 2001
- Cdc7 is required throughout the yeast S phasetoactivatereplication originsGenes & Development, 1998
- DNA double-strand-break sensitivity, DNA replication, and cell cycle arrest phenotypes of Ku-deficient Saccharomyces cerevisiaeProceedings of the National Academy of Sciences, 1997
- Structure, subnuclear distribution, and nuclear matrix association of the mammalian telomeric complex.The Journal of cell biology, 1996
- The Ku-like protein from Saccharomyces cerevisiae is required in vitro for the assembly of a stable multiprotein complex at a eukaryotic origin of replication.Proceedings of the National Academy of Sciences, 1996
- Multiple determinants controlling activation of yeast replication origins late in S phase.Genes & Development, 1996
- A position effect on the time of replication origin activation in yeastCell, 1992
- Time of replication of yeast centromeres and telomeresCell, 1988