Catecholamine-sensitive adenylate cyclase of caudate nucleus and cerebral cortex. Effects of guanine nucleotides

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Abstract
GTP and GMP-P(NH)P (guanyl-5''-yl imidodiphosphate) increased the stimulation of [rat] neural adenylate cyclase by dopamine and noradrenaline [norepinephrine]. GMP-P(NH)P had a biphasic effect on the enzyme activity. Preincubation of membranes with GMP-P(NH)P activated the enzyme by a process dependent on time and temperature. Catecholamines increased the speed and the extent of this activation. Membrane fractions contained high- and low-affinity sites for GMP-P(NH)P binding: this binding was due to protein(s) of the membrane preparations. Low-affinity-site binding of GMP-P(NH)P appeared to be related to the stimulatory effect on the adenylate cyclase activity.

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