Insulin induces translocation of glucose transporter GLUT4 to plasma membrane caveolae in adipocytes

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Abstract
SPECIFIC AIMSThe insulin receptor is localized to and signals in caveolae, and insulin signaling for metabolic control depends on intact caveolae. We earlier reported that in response to insulin, an initial rapid translocation of GLUT4 to the plasma membrane was followed by a slower transition of transporters to a detergent-insoluble plasma membrane fraction, coinciding with the increase in glucose uptake [Mol. Med., vol 2, p. 367 (1996)], which prompted us to examine the detailed localization of GLUT4 in the plasma membrane with a focus on caveolae. PRINCIPAL FINDINGS1. Cellular localization of GLUT4 in the plasma membrane in relation to caveolin as examined by immunofluorescence microscopyWe used double-immunofluorescence labeling to investigate the cellular localization of GLUT4 in relation to the caveolae marker protein caveolin in the plasma membrane of 3T3-L1 adipocytes. We sonicated the cells to expose the inner surface of the plasma membrane and fixed the membranes before antibody labeling. Fluore...
Funding Information
  • Svenska Sällskapet för Medicinsk Forskning
  • Stiftelsen för Strategisk Forskning
  • Medicinska Forskningsrådet (12157)