Systolic Effect by Sulfhydryl Reagents

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Abstract
It was assumed that the [long dash]SIT groups of cysteine and glutathione were responsible for preventing the systolic standstill of the isolated frog heart caused by angelicalactones and t-butyl hydrogen peroxide. Consequently, the following reagents which combine with SH groups ''of SH-containing enzymes were tested for their systolic standstill producing effect. Porphyrindin (2.5 X 10-3 M) caused standstill in approx. 30 mins. The effect was prevented if it was dissolved in 6.5 X 10-3 M glutathione. Ortho-iodosobenzoate (8.3 X 10-4 M) caused standstill in about 20 mins. Iodoacetamide (5.3 X 10-3 M) caused standstill in about 10 mins. p-Chloromercuric benzoate (1 X 10-6 to 1 X 10-4) caused depression and diastolic arrest; 5.6 X 10-6 M caused a 1-min. depression plus a gradual though incomplete systolic arrest. Complete systolic arrest was obtained when the heart was connected with an electrical circuit. 4.2 X 10-3 M phenarsine oxide HCl also produced stoppage before achieving systolic standstill, but complete arrest was achieved when a Cu wire was placed in the cannula 40-60 mins. before applying the drug.

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