Factors Affecting the Estimation of Concentration of Sperm in Ram's Semen by the Photoelectrometric Method

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Abstract
Ejaculates from Rambouillet, Columbia and Targhee rams were used to measure the effects of various factors on estimation of conc, with a Klett-Sommerson colorimeter. Chlora-zene was the diluter of choice, despite its low opacity and change upon prolonged standing, because it alone dissolved clumps which were prevalent in ram''s semen. A dilution of 1:200 gave readings in the optimum range of the colorimeter''s scale, and a lower error of estimate. Scores for estimating visible debris in the samples effected highly significant improvement in estimation of cone. Residual turbidity, after centrifugation of diluted samples, was not a suitable estimate of debris. Semen with the debris removed gave a regression line through the point of origin. Two brands of diluting pipettes exhibited significant differences in volume and ability to drain; however, the variability of individual pipettes was similar. The opt. technique (chlorazene at 1:200 with scoring of debris) gave a standard error of estimate of 38.1 (X 107 sperm per ml.). The error due to workers and instruments variability was negligible. The sampling and dilution errors for the colorimeter and hemacytometer were 19.7 and 21.4, respectively, but the error for the hemacytometer at the working mean was increased to 26.7 due to the Poisson distributional error of the small volume measured. The error due to non-scorable debris and heterogeneity of sample was 34.1.
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