On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli
- 1 October 1971
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 108 (1) , 375-85
- https://doi.org/10.1128/jb.108.1.375-385.1971
Abstract
A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with 3 H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either 3 H-oleate or 3 H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when 3 H-diaminopimelate is used as the marker.Keywords
This publication has 53 references indexed in Scilit:
- Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coliBiochemical and Biophysical Research Communications, 1970
- Growth of the Bacterial CellNature, 1970
- Studies on membrane synthesis in Bacillus megaterium KMJournal of Molecular Biology, 1970
- Membrane synthesis in Bacillus subtilis: II. Integration of membrane proteins in the absence of lipid synthesisJournal of Molecular Biology, 1970
- Deoxynucleoside-sensitive mutants ofSalmonella typhimuriumMolecular Genetics and Genomics, 1968
- Genetic control of the l-α-glycerophosphate system in Escherichia coliJournal of Molecular Biology, 1968
- Lethal Unbalanced Growth in BacteriaNature, 1967
- A cell-membrane bound fraction of bacterial DNABiochemical and Biophysical Research Communications, 1965
- The organization and replication of deoxyribonucleic acid in thymine-deficient strains of Escherichia coliBiochimica et Biophysica Acta, 1959
- Sur la biosynthese de la β-galactosidase (lactase) chez Escherichia coli. La specificite de l'inductionBiochimica et Biophysica Acta, 1951