Desaminierung und reduktive Desaminierung von (2-Amino-5,6-dimethylbenzimidazolyl)cobamid. Darstellung von (2-Hydroxy-5,6-dimethylbenzimidazolyl)cobamid und (5,6-Dimethyl-[2-14C]benzimidazolyl)cobamid
- 1 January 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (1) , 137-150
- https://doi.org/10.1515/bchm2.1984.365.1.137
Abstract
(2-Amino-5,6-dimethylbenzimidazolyl)cobamide is transformed to (2-hydroxy-5,6-dimethylbenzimidazolyl)cobamide by nitrous acid. Exchange of the NH2-group by hydrogen with nitrous acid/hypophosphorous acid yields vitamin B12. This reaction completes a cyclic vitamin B12 .fwdarw. [carboxy(2-cyanoamino-4,5-dimethylphenyl)amino]cobamide .fwdarw. (2-amino-5,6-dimethylbenzimidazolyl)cobamide .fwdarw. vitamin B12, which allows chemical 14C-labeling of vitamin B12. In this procedure cyanogen bromide, which is necessary for the first step, was labeled with [14C]cyanide. By the following reactions a vitamin B12 was formed in which C-2 of the 5,6-dimethylbenzimidazole moiety is labeled.This publication has 10 references indexed in Scilit:
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