The apoptosis‐associated protein BNIPL interacts with two cell proliferation‐related proteins, MIF and GFER
Open Access
- 20 March 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 540 (1-3) , 86-90
- https://doi.org/10.1016/s0014-5793(03)00229-1
Abstract
Bcl‐2/adenovirus E1B 19 kDa interacting protein 2‐like, BNIP‐2‐like (BNIPL) is a recently cloned and characterized apoptosis‐associated protein that shares 72% homology with BNIP‐2. It is highly expressed in human placenta and lung. A yeast two‐hybrid system was used to obtain two BNIPL‐interacting proteins, MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1 (Saccharomyces cerevisiae)‐like). The interactions were confirmed by glutathione S‐transferase pull‐down assay in vitro and co‐immunoprecipitation assay in vivo. Colony formation assay and cell proliferation test suggest that overexpression of BNIPL could inhibit the growth of BEL‐7402 cells. These findings suggest that BNIPL may physically bind to cell proliferation‐related proteins, MIF and GFER.Keywords
This publication has 18 references indexed in Scilit:
- The BNIP-2 and Cdc42GAP Homology/Sec14p-like Domain of BNIP-Sα Is a Novel Apoptosis-inducing SequencePublished by Elsevier ,2002
- Death receptors couple to both cell proliferation and apoptosisJournal of Clinical Investigation, 2002
- Proliferation, cell cycle and apoptosis in cancerNature, 2001
- The BNIP-2 and Cdc42GAP Homology Domain of BNIP-2 Mediates Its Homophilic Association and Heterophilic Interaction with Cdc42GAPJournal of Biological Chemistry, 2000
- Evidence for a Novel Cdc42GAP Domain at the Carboxyl Terminus of BNIP-2Journal of Biological Chemistry, 2000
- Cell Proliferation and Apoptosis in the Immune System in the ElderlyImmunologic Research, 2000
- Cell proliferation and apoptosisCurrent Opinion in Cell Biology, 1999
- THE CENTRAL EFFECTORS OF CELL DEATH IN THE IMMUNE SYSTEMAnnual Review of Immunology, 1999
- Apoptosis in the Pathogenesis and Treatment of DiseaseScience, 1995
- Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteinsCell, 1994