DETERMINATION OF BASE RATIOS OF SIX RIBONUCLEIC ACID BACTERIOPHAGES SPECIFIC TO ESCHERICHIA COLI

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Abstract
A method is described for the isolation of single-stranded-RNA coliphages. Two of the six RNA coliphages investigated were new strains. The base ratios of six RNA coliphages were determined by labelling the host bacterium with [ 32P]. phosphate, purification of the radioactive coliphages and separation of 2[image], 3[image]-ribonucleotides liberated by alkaline hydrolysis of the coliphage RNA. All six of the coliphages were morphologically similar, contained single-stranded RNA, and had sedimentation coefficient 80 [plus or minus] 5s. The six RNA coliphages fell into two distinct groups, both serologically and in terms of their RNA base ratios.