Virus detection using nanoelectromechanical devices
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- 27 September 2004
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 85 (13) , 2604-2606
- https://doi.org/10.1063/1.1794378
Abstract
We have used a resonating mechanical cantilever to detect immunospecific binding of viruses, captured from liquid. As a model virus, we used a nonpathogenic insect baculovirus to test the ability to specifically bind and detect small numbers of virus particles. Arrays of surface micromachined, antibody-coated polycrystalline silicon nanomechanical cantilever beams were used to detect binding from various concentrations of baculoviruses in a buffer solution. Because of their small mass, the 0.5μm×6μm cantilevers have mass sensitivities on the order of 10−19g∕Hz, enabling the detection of an immobilized AcV1 antibody monolayer corresponding to a mass of about 3×10−15g. With these devices, we can detect the mass of single-virus particles bound to the cantilever. Resonant frequency shift resulting from the adsorbed mass of the virus particles distinguished solutions of virus concentrations varying between 105 and 107pfu∕ml. Control experiments using buffer solutions without baculovirus showed small amounts (<50attograms) of nonspecific adsorption to the antibody layer.Keywords
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