Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR
Open Access
- 1 August 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (8) , 3841-3843
- https://doi.org/10.1128/jcm.42.8.3841-3843.2004
Abstract
Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC , i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.Keywords
This publication has 26 references indexed in Scilit:
- Rapid and Specific Detection of Escherichia coli Clonal Group A by Gene-Specific PCRJournal of Clinical Microbiology, 2004
- Global Molecular Epidemiology of the O15:K52:H1 Extraintestinal Pathogenic Escherichia coli Clonal Group: Evidence of Distribution beyond EuropeJournal of Clinical Microbiology, 2002
- Widespread Distribution of Urinary Tract Infections Caused by a Multidrug-ResistantEscherichia coliClonal GroupNew England Journal of Medicine, 2001
- Ongoing Horizontal and Vertical Transmission of Virulence Genes and papA Alleles among Escherichia coli Blood Isolates from Patients with Diverse-Source BacteremiaInfection and Immunity, 2001
- Extended Virulence Genotypes ofEscherichia coliStrains from Patients with Urosepsis in Relation to Phylogeny and Host CompromiseThe Journal of Infectious Diseases, 2000
- Accommodating Error Analysis in Comparison and Clustering of Molecular FingerprintsEmerging Infectious Diseases, 1998
- Variability in presence–absence scoring of AP PCR fingerprints affects computer matching of bacterial isolatesJournal of Microbiological Methods, 1997
- A comparative study of nosocomial and community-acquired strains of Escherichia coli causing bacteraemia in a Danish University HospitalJournal of Hospital Infection, 1995
- Amplification methods for epidemiologic investigations of infectious diseasesJournal of Microbiological Methods, 1995
- Increased resistance to quinolone in Catalonia, SpainDiagnostic Microbiology and Infectious Disease, 1993