Polypeptide Synthesis Catalysed by Components of Pichinde Virus Disrupted by Detergent
- 1 July 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 55 (1) , 213-217
- https://doi.org/10.1099/0022-1317-55-1-213
Abstract
Pichinde virus preparations were investigated for ribosomal components and associated activities. After detergent treatment and fractionation, viral components were assayed for polypeptide synthesis, elongation of nascent polypeptide chains and mRNA activity. These subviral particles could synthesize polypeptides when exogenous mRNA template, aminoacyl-tRNA, translation factors, GTP and appropriate cations were added. Undisrupted, whole virions could not synthesize polypeptide; disruption and subsequent centrifugation of subviral ribosomes was a prerequisite for the biosynthetic activity described.This publication has 5 references indexed in Scilit:
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- Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reactionArchives of Biochemistry and Biophysics, 1968