Complete complementary DNA-derived amino acid sequence of canine cardiac phospholamban.
Open Access
- 1 January 1987
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 79 (1) , 301-304
- https://doi.org/10.1172/jci112799
Abstract
Complementary DNA (cDNA) clones specific for phospholamban of sarcoplasmic reticulum membranes have been isolated from a canine cardiac cDNA library. The amino acid sequence deduced from the cDNA sequence indicates that phospholamban consists of 52 amino acid residues and lacks an amino-terminal signal sequence. The protein has an inferred mol wt 6,080 that is in agreement with its apparent monomeric mol wt 6,000, estimated previously by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Phospholamban contains two distinct domains, a hydrophilic region at the amino terminus (domain I) and a hydrophobic region at the carboxy terminus (domain II). We propose that domain I is localized at the cytoplasmic surface and offers phosphorylatable sites whereas domain II is anchored into the sarcoplasmic reticulum membrane.This publication has 30 references indexed in Scilit:
- Concerted regulation of cardiac sarcoplasmic reticulum calcium transport by cyclic adenosine monophosphate dependent and calcium-calmodulin-dependent phosphorylationsBiochemistry, 1979
- Mechanism of the stimulation of Ca2+-dependent ATPase of cardiac sarcoplasmic reticulum by adenosine 3‘:5‘-monophosphate-dependent protein kinase. Role of the 22,000-dalton protein.Journal of Biological Chemistry, 1979
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Phosphorylation of a 22,000-dalton component of the cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase.Journal of Biological Chemistry, 1975
- The Stimulation of Calcium Transport in Cardiac Sarcoplasmic Reticulum by Adenosine 3′:5′-Monophosphate-dependent Protein KinaseJournal of Biological Chemistry, 1974
- Adenosine 3′:5′-Monophosphate-dependent Protein Kinase-catalyzed Phosphorylation Reaction and Its Relationship to Calcium Transport in Cardiac Sarcoplasmic ReticulumJournal of Biological Chemistry, 1974
- [30] Purification of messenger RNA and heterogeneous nuclear RNA containing poly(A) sequencesPublished by Elsevier ,1974
- Use of Helical Wheels to Represent the Structures of Proteins and to Identify Segments with Helical PotentialBiophysical Journal, 1967