Enhancement of Tobacco Mosaic Virus Spread in Mechanically Inoculated Leaves by Pre-incubation at a Nonpermissive Temperature

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Abstract
In tobacco [Nicotiana tabacum] leaves mechanically inoculated with TMV [tobacco mosaic virus] and preincubated at temperatures nonpermissive for virus synthesis, the rate of TMV synthesis upon shift to a permissive temperature was markedly greater than that in control leaves. Preincubation of infected leaves at 10-12.degree. C inhibited virus synthesis most effectively, and subsequent transfer to 25.degree. C produced the most rapid rate of virus multiplication. The optimum duration of preincubation was 10 days. In leaves that were preincubated at 12.degree. C for 10 days after mechanical inoculation, infectivity increased exponentially from the time of the temperature shift until about 16 h at 25.degree. C and linearly from 16 h until 3-4 days at 25.degree. C when the maximum level of virus was attained. The maximum level of virus accumulated in control leaves only after 10 days. Preincubation at 12.degree. C enhanced cell-to-cell spread of virus either while at the nonpermissive temperature or immediately after the temperature shift.

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