Rules for RNA recognition of GNRA tetraloops deduced by invitro selection: comparison with invivo evolution

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Abstract
Terminal loops with a GNRA consensus sequence are a prominent feature of large self‐assembling RNA molecules. In order to investigate tertiary interactions involving GNRA loops, we have devised an in vitro selection system derived from a group I ribozyme. Two selections, destined to isolate RNA sequences that would recognize two of the most widespread loops (GUGA and GAAA), yielded variants of previously identified receptors for those loops, and also some yet unrecognized, high‐affinity binders with novel specificities towards members of the GNRA family. By taking advantage of available crystal structures, we have attempted to rationalize these results in terms of RNA–RNA contacts and to expose some of the structural principles that govern GNRA loop‐mediated tertiary interactions; the role of loop nucleotide 2 in ensuring specific recognition by receptors is emphasized. More generally, comparison of the products of in vitro and natural selection is shown to provide insights into the mechanisms underlying the in vivo evolution of self‐assembling RNA molecules.