Stabilization of the Photosynthetic Activities of Isolated Spinach Chloroplasts during Prolonged Ageing
Open Access
- 1 August 1976
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 31 (7-8) , 452-455
- https://doi.org/10.1515/znc-1976-7-820
Abstract
Isolated spinach chloroplasts (type A complete) were used to study the changes in the photo-chemical activities upon storage in order to establish optimum conditions for prolonged storage. Chloroplasts stored at - 5 °C were found to retain over 70% of their photosynthetic electron transport from H2O even after 5 days. Increases in the level of state 2 electron transport (due to uncoupling) with concomitant loss of state 3 phosphorylation activity was observed during the initial period of ageing. Addition of 1% bovine serum albumin decreased the level of uncoupling and maintained the phosphorylation activity for a longer period. Chloroplasts stored at 77 °K maintained their phosphorylation capacity for a period of 10 days even after repeated freezing and thawing. Photosystem I activity was found to be more stable over the whole ageing period of 15 days. Loss of plastoquinone may be responsible for the decrease in electron transport between photosystems II and I.Keywords
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