A single amino acid mutation in a protein antigen abrogates presentation of certain T cell determinants.
Open Access
- 1 December 1989
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 170 (6) , 2171-2176
- https://doi.org/10.1084/jem.170.6.2171
Abstract
Nase-specific T cell recognize the 86-100 peptide in association with B10.A APC. Clone N40 recognizes the 86-100 peptide in association ith B10.A (E.alpha.k E.beta.k) and B10.A (5R) (E.alpha.k E.beta.b) APCs. We demonstrate here that a single amino acid substitution in the staphylococcal nuclease protein alters the structure of the processed peptide such that the T cell epitope recognized by clone N40 was only available for recognition in conjunction with B10.A (5R) but not the B10.A APCs. Other Nase-specific T cells recognize the mutant nuclease, and a synthetic peptide corresponding to the immunodominant region of the mutant protein was stimulatory for all the Nase-specific T cells. These results suggest that the mutation either affects the processing of the protein into antigenic peptides or affects the conformation of the processed fragment differently from that of the peptide.This publication has 17 references indexed in Scilit:
- Class I cross-restricted T cells reveal low responder allele due to processing of viral antigenNature, 1989
- The activation of pigeon cytochrome c-specific T cell hybridomas by antigenic peptides is influenced by non-native sequences at the amino terminus of the determinant.The Journal of Immunology, 1988
- Structural characteristics of an antigen required for its interaction with Ia and recognition by T cellsNature, 1987
- Identification of the T-cell and Ia contact residues of a T-cell antigenic epitopeNature, 1987
- The T cell repertoire for recognition of a phylogenetically distant protein antigen. Peptide specificity and MHC restriction of staphylococcal nuclease-specific T cell clones.The Journal of Experimental Medicine, 1986
- Amino acid residues distinct from the determinant region can profoundly affect activation of T cell clones by related antigens.The Journal of Immunology, 1986
- Binding of immunogenic peptides to Ia histocompatibility moleculesNature, 1985
- T-Lymphocyte Recognition of Antigen in Association with Gene Products of the Major Histocompatibility ComplexAnnual Review of Immunology, 1985
- Isolation and characterization of a cDNA clone for the murine I-E beta polypeptide chain.Proceedings of the National Academy of Sciences, 1983
- Decrease in macrophage antigen catabolism caused by ammonia and chloroquine is associated with inhibition of antigen presentation to T cells.Proceedings of the National Academy of Sciences, 1982