THE PRACTICAL IDENTIFICATION OF ENDOTHELIAL LEUKOCYTES IN DIFFERENTIAL BLOOD COUNTING
Open Access
- 1 August 1918
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of internal medicine (1908)
- Vol. XXII (2) , 157-159
- https://doi.org/10.1001/archinte.1918.00090130031002
Abstract
A method for the identification of endothelial leukocytes in the peripheral blood has been reported by one of us.1This method, as was pointed out in its description, is not well adapted to routine work on account of the impossibility of bringing all cells into contact with the carbon particles of the suspension employed to determine the phagocytic properties of the leukocytes. The purpose of this paper is to record the application to blood films of an indophenol staining reaction which brings out characteristic cytoplasmic granules in the endothelial leukocytes. Leukocytes Granule Stain. —To apply the stain, a blood film made in the usual way on a 22-mm. square coverglass is covered for one half minute with 5 drops of an alphanaphthol-methyl-violet solution in order to fix the preparation; the alcoholic stain is then diluted with an equal amount of distilled water, and the dilute stain allowed to act forThis publication has 0 references indexed in Scilit: