INDIUM-111-LABELED AUTOLOGOUS LEUKOCYTES IN MAN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 18  (10) , 1014-1021
Abstract
Autologous leukocytes were isolated, labeled with 111In, and administered to 15 patients suspected of inflammatory disease. The stability of the label was demonstrated and the in vivo kinetics and distribution of the labeled cells studied. The distribution is influenced by the type and viability of the cells separated by 3 different techniques. Generally, there was initial accumulation of radioactivity in the lungs; approximately half of this cleared in 15 min and the remainder slowly. Twenty-five to 50% of the radioactivity subsequently distributed in the spleen, liver and bone marrow, and these did not show significant change with time up to 48 h post injection. The 111In radioactivity administered as labeled leukocytes free from erythrocytes cleared from the circulating blood with a half-time of 7.5 h. In 3 of 15 patients, the suspicion of inflammatory disease was not confirmed, and in these a normal distribution of radioactivity was observed. In the remaining 12 patients, focal accumulation of radioactivity was detectable within 4-24 h after administration, and subsequent confirmation of sepsis was obtained. From 3 such patients, samples of abscesses were recovered which showed markedly higher radioactivity than that in the same weight of blood.