SEPARATION OF LYMPHOCYTE SUBPOPULATIONS IN CARP CYPRINUS-CARPIO L BY MONOCLONAL-ANTIBODIES - IMMUNOHISTOCHEMICAL STUDIES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 48  (1) , 165-175
Abstract
Lymphoid cell populations in various organs of the carp C. carpio L. were investigated using a series of mouse monoclonal antibodies raised against carp thymocytes and carp serum Ig. Clones were designated as Ig+ T+, Ig+ T- or Ig- T+ on the basis of the reactivity with thymocytes and serum Ig in the enzyme-linked immunosorbent assay screening. Their reaction to the lymphoid organs of carp was investigated on cryostat sections and cytocentrifuge slides using immunoperoxidase techniques. Ig+ T+ clones were subdivided into those that stained reticular fibers around blood vessels in various organs (R+) and those that did not (R-). The former stained most thymocytes and most peripheral lymphocytes as well as plasma cells; the latter did not stain cortical thymocytes and some peripheral lymphocytes. Ig+ T- clones were negative for thymocytes but positive for plasma cells and a certain population of peripheral lymphocytes. Ig- T+ clones reacted similarly to Ig+ T+ R- clones. Fish lymphoid cell populations apparently can be distinguished based on differences in cell surface and/or cytoplasmic determinants. The monoclonal antibodies described can be used for further structural analysis of the determinants and for functional separation of T- and B-like cells in the lower vertebrates.