Transmission and Multiplication of Newcastle Disease Virus (NDV) in Brains of Suckling Mice

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Abstract
Suckling mice of the Swiss Albino strain are readily susceptible to Newcastle disease of poultry on intracerebral inoculation of virus. Ten successive passages were carried out by means of brain-to-brain transfers, starting with 3-day-old mice. Similar results were obtained with an Australian and the California (No. 11,914) strains of Newcastle disease virus. Afflicted mice had humped backs and used their hind legs stiffly. Proof that Newcastle disease virus was actually transferred was obtained by inoculation of eggs with infected mouse brains. Egg fluids harvested in 42 hrs. were successfully used in antihemagglutination tests using a standard NDV-immune serum. Infected mouse brain also produced a characteristic picture of Newcastle disease on intracerebral inoculation of chickens. Using infected brains of suckling mice, a few passages of NDV have been successful in adult mice.