PURINE COMPOUNDS IN MITOCHONDRIA - A QUANTITATIVE-EVALUATION
- 1 January 1989
- journal article
- research article
- Vol. 48 (1) , 57-61
Abstract
Applying a recently developed reverse-phase ion-pair HPLC technique we determined purine compounds in acid extracts of mitochondria isolated from rat and mouse liver, rat and cortex, and hepatoma. Intramitochondrial purine nucleosides and bases were evaluated quantitatively for the first time. All mitochondria examined contain significant amounts of adenosine, guanosine, inosine, guanine, and hypoxanthine. Moreover, IMP was identified in all extracts. Adenine was detected in traces only. These results are in accordance with the concept of mitochndrial metabolism of purine compounds.This publication has 14 references indexed in Scilit:
- Increased Nucleoside Concentrations in Tumors as Markers of Tiazofurin ActionNucleosides, Nucleotides and Nucleic Acids, 1986
- The Separation of the Major Ribonucleotides, Nucleosides, and Bases in Reverse-Phase Ion-Pair ChromatographyJournal of Liquid Chromatography, 1986
- Influence of Isolation Media on the Preservation of Mitochondrial FunctionsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Unusual compartmentation of precursors for nuclear and mitochondrial DNA in mouse L cells.Journal of Biological Chemistry, 1982
- Regulation of Deoxynucleoside Kinase Activities in Rat Liver MitochondriaEnzyme, 1982
- Adenine nucleotide efflux in mitochondria induced by inorganic pyrophosphateBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1982
- Regulation of the mitochondrial adenine nucleotide pool sizeArchives of Biochemistry and Biophysics, 1981
- Intramitochondrial adenine nucleotides and energy-linked functions of heart mitochondriaAmerican Journal of Physiology-Heart and Circulatory Physiology, 1981
- In vitro alteration of the size of the liver mitochondrial adenine nucleotide pool: Correlation with respiratory functionsArchives of Biochemistry and Biophysics, 1980
- Relationship between 5-Nucleotidase, Adenosine Deaminase, AMP Deaminase, ATP-(Mg^2+)-ase Activities and dTMP Kinase Activity in Rat Liver MitochondriaEnzyme, 1979