Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 60 (1) , 85-89
- https://doi.org/10.1007/bf00495731
Abstract
Summary Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4° C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 μ, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 μ. The simultaneous coupling azo dye method using the substrates sodium α-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.Keywords
This publication has 8 references indexed in Scilit:
- Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple StainsStain Technology, 1976
- A simple method of preparing 1-2 μm sections of large tissue blocks using glycol methacrylateJournal of Microscopy, 1974
- Freeze-drying and embedding in glycol methacrylate (GMA)Histochemistry and Cell Biology, 1974
- The significance of enzyme histochemical patterns in carcinomas of the large intestine in manGut, 1973
- Adenocarcinoma, adenomas and polyps of the colon:Histochemical studyCancer, 1968
- The Distribution of Certain Enzyme Systems in the Normal Human Gastrointestinal TractGastroenterology, 1963
- THE DEMONSTRATION OF ALKALINE PHOSPHATASE ACTIVITY IN FROZEN-DRIED MOUSE GUT IN THE ELECTRON MICROSCOPEJournal of Histochemistry & Cytochemistry, 1963
- Histochemical Comparison of Naphthol AS-Phosphates for the Demonstration of PhosphatasesJNCI Journal of the National Cancer Institute, 1958