Molecular analysis of theRhizobiumgenes involved in the induction of nitrogen-fixing nodules on legumes
- 24 September 1987
- journal article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. B, Biological Sciences
- Vol. 317 (1184) , 193-207
- https://doi.org/10.1098/rstb.1987.0057
Abstract
Recent developments in the molecular genetics ofRhizobium spp. are presented, and the use of mutant bacterial strains to determine which properties are required for symbiotic nitrogen fixation and nodulation of legumes is described. Both the lipopolysaccharide and the exopolysaccharide ofRhizobium spp. are implicated in infection. Recent studies have identified several genes involved in the early steps of this process and in the determination of host-range specificity. Analysis of their products has given some indications of their functions. The expression of most of these nodulation (nod) genes is controlled by the regulatory genenodD, which is itself expressed constitutively, whereas other nod genes are transcribed only when the cells are exposed to compounds present in the rhizosphere of legumes. These compounds were identified as various flavones and flavanones. Other plant-specified aromatic molecules, such as isoflavonoids, antagonize this induction.Keywords
This publication has 43 references indexed in Scilit:
- Analysis of Lipopolysaccharide from Root Nodule Bacteroids of Rhizobium leguminosarum Using Monoclonal AntibodiesMicrobiology, 1986
- Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciensProceedings of the National Academy of Sciences, 1986
- A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen XanthomonasMolecular Genetics and Genomics, 1986
- Nucleotide Sequence ofRhizobium meliloti1021 Nodulation Genes:nodDIs Read Divergently fromnodABCDNA, 1985
- Nitrogen fixation specific regulatory genes ofKlebsiella pneumoniaeandRhizobium melilotishare homology with the general nitrogen regulatory genentrCofK. pneumoniaeNucleic Acids Research, 1985
- Structural relationships among rhizobium meliloti symbiotic promotersCell, 1983
- The cyclic structure of β-d-(1→2)-linked d-glucans secreted by Rhizobia and AgrobacteriaCarbohydrate Research, 1983
- Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in PseudomonasGene, 1981
- The genetic analysis of Rhizobium in relation to symbiotic nitrogen fixationHeredity, 1980
- Transfer of the drug-resistance transposon Tn5 to RhizobiumNature, 1978