Pigment‐Protein Complexes from the Photosynthetic Membrane of the Cyanobacterium Synechocystis sp. PCC 6803
Open Access
- 1 December 1995
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 234 (2) , 459-465
- https://doi.org/10.1111/j.1432-1033.1995.459_b.x
Abstract
Photosystem I and II core complexes were resolved in a single step from the thylakoid membrane of Synechocystis sp. PCC 6803 by using a mild solubilization procedure in dodecyl beta-D-maltoside and Deriphat/PAGE. For each photosystem, two green bands were obtained containing oligomeric and monomeric forms of the core complexes of either photosystem. The oligomers are likely to be trimers in the case of photosystem I and dimers for photosystem II. The absorption spectra, polypeptide and pigment composition of green bands corresponding to either photosystem I or photosystem II were identical for monomeric and oligomeric forms. The cytochrome b-559 content of photosystem II was evaluated to be one cytochrome b-559/reaction centre both in the monomeric and dimeric forms. Two new 15-kDa and 22-kDa carotenoid-binding protein were isolated and their polypeptides purified to homogeneity.Keywords
This publication has 57 references indexed in Scilit:
- Comparison of Cytochrome b-559 Content in Photosystem II Complexes from Spinach and Synechocystis Species PCC 6803Biochemistry, 1994
- Three-dimensional structure of system I of photosynthesis at 6 Å resolutionNature, 1993
- Light‐induced degradation of D2 protein in isolated photosystem II reaction center complexFEBS Letters, 1992
- Characterization of a 41 kDa photoinhibition adduct in isolated photosystem II reaction centresFEBS Letters, 1992
- New evidence suggests that the initial photoinduced cleavage of the D1‐protein may not occur near the PEST sequenceFEBS Letters, 1991
- Characterisation of a PS II reaction centre isolated from the chloroplasts ofPisum sativumFEBS Letters, 1987
- Isolation and Characterization of a Carotenoid-Associated Thylakoid Protein from the Cyanobacterium Anacystis nidulans R2Plant Physiology, 1987
- Evidence for a trimeric organization of the photosystem I complex from the thermophilic cyanobacteriumSynechococcussp.FEBS Letters, 1987
- Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activityFEBS Letters, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970