Effect of storage conditions of the performance of bismuth sulfite agar

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Abstract
Refrigerated storage of bismuth sulfite agar plates for up to 4 days did not adversely affect growth and colonial characteristics of selected Salmonella strains [S. newport, S. infantis, S. typhimurium, S. typhi, S. agona]. Incubation of inoculated plates for 48 h favored the development of more salmonellae with typical morphology. Inoculated plates of freshly poured medium incubated for 48 h gave recoveries similar to those on refrigerated plates and showed a high selectivity against Citrobacter freundii and Proteus vulgaris, organisms which mimic the colonial characteristics of Salmonella on this medium. The use of bismuth sulfite plates stored at room temperature for > 2 days should be avoided.