Binding of methylmercuric hydroxide by 4-thiouridine of N-formylmethionyl-tRNA from Escherichia coli
- 1 June 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 54 (6) , 583-587
- https://doi.org/10.1139/o76-086
Abstract
The binding of methylmercuric hydroxide to N-formylmethionyl-tRNA of E. coli was studied. In the absence of Mg2+, methylmercury binds strongly to 4-thiouridine in the 8th position from the 5'' end; the dissociation constant is (1.0 .+-. 0.2) .times. 10-5 M and the forward and reverse rate constants for binding are (5.3 .+-. 0.5) .times. 104 M-1 s-1 and 0.38 .+-. 0.02 s-1, respectively. The presence of Mg2+ prevents this binding, presumably through some conformational change induced in the structure of N-formylmethionyl-tRNA.This publication has 4 references indexed in Scilit:
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- Studies on Tyrosine Transfer Ribonucleic Acid, a Sulfur-rich Species from Escherichia coliJournal of Biological Chemistry, 1967
- Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- Complexing and denaturation of DNA by methylmercuric hydroxideJournal of Molecular Biology, 1966